WormBase Tree Display for Construct: WBCnstr00019383
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| WBCnstr00019383 | Public_name | pJM1 | |
|---|---|---|---|
| Summary | [Punc-17 small:ChR2::YFP] | ||
| Driven_by_gene | WBGene00006756 | ||
| Fusion_reporter | YFP | ||
| Other_reporter | ChR2 | ||
| Construction_summary | pJM1 contains a short unc-17 promoter (Punc-17S) driving ChR2:YFP. It was constructed using LR clonase to recombine pLR159, containing Punc-17S, with pLR167. pLR159 was created using primers attb1unc17pfor and attb2unc17pbac to PCR amplify a short (3678 bp) version of the unc-17 promoter from genomic DNA and recombining the promoter with pDG15 using BP clonase II. | ||
| Used_for | Transgene_construct | WBTransgene00020058 | |
| Reference | WBPaper00045365 |
