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WormBase Tree Display for Construct: WBCnstr00038229

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Name Class

WBCnstr00038229Summary[Punc-119::GFP::CDC-25.4::unc-54 3' UTR]
Driven_by_geneWBGene00006843
GeneWBGene00000389
Fusion_reporterGFP
Construction_summaryPmyo-3::mCherry::unc-54 3' UTR (pCFJ104) was used to generate neuronal promoter-driven cdc-25.4 rescue constructs. First, pCFJ104 DNA was linearized by inverse PCR using primers designed at the end of Pmyo-3 and the beginning of unc-43 3' UTR sequences. Then, a gfp::cdc-25.4 fusion sequence, which was PCR-amplified from the Pcdc-25.4::gfp::cdc-25.4::cdc-25.4 3' UTR translational reporter construct, was inserted into the linearized pCFJ104 DNA by the In-Fusion reaction. The myo-3 promoter sequence was replaced with the promoter sequences of unc-119 (2.3 kb) (Maduro and Pilgrim 1995).
Used_forTransgene_constructWBTransgene00023626
ReferenceWBPaper00050301