WormBase Tree Display for Construct: WBCnstr00038229
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WBCnstr00038229 | Summary | [Punc-119::GFP::CDC-25.4::unc-54 3' UTR] | |
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Driven_by_gene | WBGene00006843 | ||
Gene | WBGene00000389 | ||
Fusion_reporter | GFP | ||
Construction_summary | Pmyo-3::mCherry::unc-54 3' UTR (pCFJ104) was used to generate neuronal promoter-driven cdc-25.4 rescue constructs. First, pCFJ104 DNA was linearized by inverse PCR using primers designed at the end of Pmyo-3 and the beginning of unc-43 3' UTR sequences. Then, a gfp::cdc-25.4 fusion sequence, which was PCR-amplified from the Pcdc-25.4::gfp::cdc-25.4::cdc-25.4 3' UTR translational reporter construct, was inserted into the linearized pCFJ104 DNA by the In-Fusion reaction. The myo-3 promoter sequence was replaced with the promoter sequences of unc-119 (2.3 kb) (Maduro and Pilgrim 1995). | ||
Used_for | Transgene_construct | WBTransgene00023626 | |
Reference | WBPaper00050301 |