WormBase Tree Display for Construct: WBCnstr00040211
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| WBCnstr00040211 | Summary | Cel-unc-54(prom)::Cel-her-1(cDNA)::Cel-her-1(3'UTR) | |
|---|---|---|---|
| Driven_by_gene | WBGene00006789 | ||
| Gene | WBGene00001842 | ||
| UTR_3 | WBGene00001842 | ||
| Construction_summary | Plasmids containing the <i>C. briggsae</i> or <i>B. malayi</i> cDNAs driven by the Ce-unc-54 promoter and followed by the Ce-her-1 3'UTR were made by three-part ligation using the vector pPD30.38 (which contains the unc-54 promoter; Fire et al. 1990). The <i>C. elegans</i> her-1 3'UTR fragment was 0.8 kb in length, starting immediately after the termination codon. The <i>C. briggsae</i> cDNA was generated by RT-PCR using the primers CB5'UTRF (located 54 bp upstream of the putative initiator AUG) and CBCE3'R. | ||
| Clone | pPD30.38 | ||
| Used_for | Transgene_construct | WBTransgene00025453 | |
| WBTransgene00025455 | |||
| Reference | WBPaper00003644 | ||
| Laboratory | BW |
