WormBase Tree Display for Interaction: WBInteraction000504523
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| WBInteraction000504523 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00006831 | Interactor_type | Trans_regulator | |
| WBGene00000149 | Interactor_type | Trans_regulated | |||
| Expr_pattern | Expr4548 | ||||
| Variation_interactor | WBVar00143874 | Interactor_type | Trans_regulator | ||
| Interaction_summary | APL-1::GFP transgenic rescue strain was crossed to the hypomorphic mutant unc-104(e1265). Interestingly, rather than observing an accumulation of the GFP fluorescence in the cell body, which traditionally results from the reduction of UNC-104 mediated vesicle transport, a dramatic decrease in the fluorescence from apl-1::gfp was found on the unc-104(e1265) background, as measured by fluorescence intensity in a set of three head inter-neurons that consistently expressed apl-1. Additionally, APL-1 fluorescence was absent from the processes of the neurons, quantified from a specific dorsal process that is consistently visible on the N2 background. Western blotting from L4 worms also detects a drop in APL-1 protein expression on the unc-104(e1265) background . qRT-PCR comparing apl-1 expression between N2 and unc- 104(e1265) backgrounds showed no differences, suggesting that the loss of APL-1::GFP is occurring at the protein level. | ||||
| Detection_method | Reporter_gene | [apl-1::gfp], translational fusion | |||
| Western | |||||
| RT_PCR | |||||
| Regulation_level | Post_transcriptional | ||||
| Regulation_result | Positive_regulate | Anatomy_term | WBbt:0003679 | ||
| Paper | WBPaper00037623 |
