WormBase Tree Display for Interaction: WBInteraction000541913

WBInteraction000541913 Interaction_type: Regulatory: Change_of_localization
  Interactor: Interactor_overlapping_gene: WBGene00000912 Interactor_type: Trans_regulated
    Molecule_interactor: WBMol:00005340 Interactor_type: Trans_regulator
  Interaction_summary: "we used daf-16:gfp worms, which contain a transgenic GFP protein fused to DAF-16, to evaluate how a-SWCNT exposure affects the nuclear translocation of DAF-16 in C. elegans. Worms previously treated with or without a-SWCNTs (500 mg/mL) were starved for 2 h to induce DAF-16 nuclear translocation [44]. Under starvation conditions, DAF-16:GFP became localized to the nucleus (Fig. 6D). As expected, a-SWCNTexposed worms displayed a 1.7-fold decrease in nuclear DAF- 16:GFP fluorescence relative to the untreated controls (Fig. 6E), indicating that a-SWCNT treatment inhibited the nuclear translocation of DAF-16 in worms."
  Detection_method: Reporter_gene: DAF-16::GFP
  Regulation_level: Post_translational
  Regulation_result: Negative_regulate: Subcellular_localization: nucleus
  Paper: WBPaper00042331
  Remark: Fig. 6de; 500 ug/mL aminated single-walled carbon nanotubes