WormBase Tree Display for Interaction: WBInteraction000542538
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| WBInteraction000542538 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00002026 | Interactor_type | Trans_regulated | |
| Transgene | WBTransgene00009056 | ||||
| Construct | WBCnstr00008745 | ||||
| WBGene00006781 | Interactor_type | Trans_regulator | |||
| Interaction_summary | "The screen for negative regulators of the HSR identified genes whose reduced expression resulted in the constitutive expression of the phsp70::gfp reporter. To ensure that these regulators activated the HSR in an HSF1-dependent manner, we employed a subsequent counter-screen using a hypomorphic hsf-1 mutant [40]. Candidate negative regulators were also tested for their ability to constitutively activate endogenous heat shock genes by qRT-PCR (Figure 1F). This strategy led to the identification of fifty-two genes that have the functional properties of negative regulators of the HSR (Table 2, Table S1)." | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [hsp-70p::gfp] | ||||
| Transgene | |||||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Does_not_regulate | Anatomy_term | WBbt:0005772 | ||
| WBbt:0005319 | |||||
| Paper | WBPaper00042355 | ||||
| Remark | Table 2, Table S1 |
