WormBase Tree Display for Expression_cluster: WBPaper00025032:PAL-1_target_genes
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| WBPaper00025032:PAL-1_target_genes | Description | Potential PAL-1 target genes. | |
|---|---|---|---|
| Species | Caenorhabditis elegans | ||
| Algorithm | Clustering, correlation to known target genes and ANOVA were used to score the PAL-1 target potential of each gene. | ||
| Reference | WBPaper00025032 | ||
| Microarray_results (162) | |||
| Gene (166) | |||
| Attribute_of | Microarray_experiment (30) | ||
| Regulation | Regulated_by_gene | WBGene00003912 | |
| Associated_with | WBProcess | WBbiopr:00000024 | |
| Remark | Clustering, correlation to known target genes and ANOVA were used to score the PAL-1 target potential of each gene. Only genes with maximum expression over time and genotype greater than the median of all genes over time and genotype (transcript abundance of thirteen RMA units) were considered as potential targets. Clusters 24, 50, 60, 140, 187, 141, 168, 85, 105, 130, 131, 144, 177, 195 and 88 were selected as potential target clusters and each gene belonging to one of these clusters was given a target score of 1. A target score of 1 was also given to genes that were either one of the ten best correlated genes with vab-7 over time and genotype or one of the 100 best correlated genes with cwn-1. Finally, model-based and quantitative ANOVA was used to assign a target score of 1-5 depending on the P-value assigned to each gene at each time point for the observed differences in expression between the three genotypes. The final target score was assigned to each gene by adding the max of the ANOVA-based model scores, the score from cluster analysis (1 or 0) and the score from correlation to known targets (1 or 0) producing a maximum score of 7. Genes with target score of 1 showed target validation efficiency of only ~25%, therefore only genes with scores of 2 and higher are included in this list. | ||
| Type: Co-expression Cluster |
