Comparisons of RMA normalized intensities for unc-4::GFP vs reference cells were statistically analyzed using Significance Analysis of Microarrays software (SAM, Stanford). A two-class unpaired analysis of the data was performed to identify genes that differ by 1.7-fold from the wildtype reference at a False Discovery Rate (FDR) of 1%. These genes were considered significantly enriched.
unc-4::GFP is expressed in 13 embryonic motor neurons. (1) I5 (pharynx), (3) SAB (retrovesicular ganglion), and (9) DA (ventral nerve cord).
Intensity values were normalized using RMA (Robust Multi-Array Analysis) available through GeneTraffic (Iobion). Comparisons of RMA normalized intensities for unc-4::GFP vs reference cells were statistically analyzed using Significance Analysis of Microarrays software (SAM, Stanford). A two-class unpaired analysis of the data was performed to identify genes that differ by >= 1.7-fold from the wildtype reference at a False Discovery Rate (FDR) of <= 1%. These genes were considered significantly enriched.