WormBase Tree Display for Microarray_experiment: WBPaper00040911:L4_ALG-2-TAG_control_IP
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WBPaper00040911:L4_ALG-2-TAG_control_IP | Microarray | GPL9815 | |
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Species | Caenorhabditis elegans | ||
Sample | Microarray_sample | WBPaper00040911:L4_ALG-2-TAG_control_IP | |
Reference | WBPaper00040911 | ||
Remark | GEO record: GSM869847 | ||
Frequency = normalized median signal intensities. The median of four individual microarray spots was used for analysis. 200 units were substracted as a global background based on an estimate of background exactly as described in PMID: 15345052. Images were processed using GenePix Pro 6 Acquisition and Analysis Microarray Software. Spot intensity data was then analysed using R version 2.13.1 and Bioconductor 2.8 packages including limma and gplots for visualisation. Normalized spot intensities were used for all data analysis. For inter-array normalisation all data were scaled based on total array intensities. Data were filtered using a cut-off of 5 fold above background. |