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| WBPicture0000007369 | Description | Figure S1. Molecular Cloning of CeRictor Mutants (A) Gene structure for lpo-6 (F29C12.3). Intron/exon boundaries are shown to scale. Below is a schematic of the LPO-6 protein; numbers reflect amino acid sequence of LPO-6 protein (there are no consensus domains in the protein as predicted by sequence homology). Sites and consequences of both mg360 and ft7 mutations are shown. (B) Multiple-species alignment of conserved, C-terminal region where mg360 allele substitution is found. Asterisk denotes the mutated residue. (C) Expression pattern of rescuing lpo-6 promoter fused to GFP in adult animal. Note exclusively intestinal expression pattern, with the exception of I1 interneuron. Image results from merge of fluorescence and DIC channels. (D) Expression pattern of rescuing lpo-6 promoter fused to GFP in early larval animal. Note both intestinal (I) and hypodermal (H) expression. Image results from merge of fluorescence and DIC channels. (E) Expression of rescuing lpo-6 promoter fused to GFP in I1 interneuron. Image results from merge of fluorescence and DIC channels. Neuron was identified as I1 due to the position of the cell body and a projection crossing the pharynx and extending anterior on the side opposite the cell body. | |||
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| Name | journal.pbio.1000060.sg001.jpg | ||||
| Crop | Crop_picture | WBPicture0000007370 | |||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2009 | ||||
| Article_URL | DOI | id | 10.1371/journal.pbio.1000060 | ||
| Journal_URL | PLoSBiology | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00032966 |
