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WBPicture0000007575Descriptionsma-9 is widely expressed. sma-9 upstream sequences (K) were fused with a GFP reporter gene. (A-C) GFP expression in wild-type transgenic animals. The transgenic animals display a strong fluorescence in the VNC, pharynx and intestine, from stages L1 (not shown) to adult (A,B). L2 stage (C) animals show expression in the seam cells that disappears after L3 (Nomarski image in D). Arrows in C and D indicate seam cell nuclei. (E,I) Immunostaining by anti-SMA-9 antibodies against the unique 70 aa C terminus present in class II isoforms. (F,J) DAPI staining of the same animals. (E,F) N2; (I,J) enlarged view of the anterior region of a wild-type late L1 stage animal. In all animals, anterior is to the left, arrowheads show pharyngeal nuclei, black arrows show intestinal nuclei and white arrows show hypodermal nuclei. (K) Different sma-9 upstream regions used to construct GFP reporters.
NameF2.large_AJ.jpg
CropCropped_fromWBPicture0000007574
DepictExpr_patternExpr2782
Expr2783
AnatomyWBbt:0003681
WBbt:0004017
WBbt:0005300
WBbt:0005753
WBbt:0005772
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2003
Article_URLDOIid10.1242/dev.00863
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00006346