WormBase Tree Display for Picture: WBPicture0000007716

WBPicture0000007716 Description: Figure 1. PKD-2 localizes to cilia and ER in male-specific sensory neurons. (A-G) Images are projected z-series obtained by laser-scanning confocal microscopy unless indicated otherwise. (A) A lateral view of an adult male expressing Ppkd-2::GFP (pkd-2 promoter driving GFP expression). GFP is expressed in four CEMs in the head, 16 ray B neurons, and the HOB neuron in the tail. Scale bar: 100 μm. (B,C) PKD-2::GFP localizes in the cell bodies (arrows), small dendritic and axonal puncta, and ciliary endings (arrowheads). Scale bars: 10 μm. (B) CEMs, (C) RnB ray (three arrows) and HOB (one arrow) neuronal cell bodies, and ciliary regions (ciliary membrane and base, arrowheads). (D,E) GFP-tagged TRAM expressed under (Ppkd-2::TRAM::GFP) localizes in cell bodies (arrows), dendritic puncta and ciliary bases (open arrowheads), but not the cilium proper. Scale bars: 10 μm. (D) CEMs, (E) HOB and RnB neurons. (F,G) GFP-KDEL ER marker driven by the pkd-2 promoter shows reticular structure in cell bodies and is excluded from ciliary and dendritic compartment of the cells. Open arrowheads indicate the absence of GFP in cilia. (H) Epifluorescence images showing overlapping expression of PKD-2::GFP and Ppkd-2::TRAM::DsRed2 in RnB cell bodies.
  Name: F1.large.jpg
  Depict: Expr_pattern: Expr4254
    Anatomy (21)
    Cellular_component: GO:0030424
      GO:0030425
      GO:0043025
      GO:0097730
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2006
    Article_URL: DOI id 10.1242/dev.02555
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00028448