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| WBPicture0000008148 | Description | Figure 1. Immunochemical analysis of VHA-11. A, detection of VHA-11 in C. elegans total lysate. A total lysate of yeast cells carrying either a vector (Vector) or the vha-11 expression plasmid (pVHA-11), and a C. elegans lysate (C. elegans) were subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, blotted onto nitrocellulose membranes, and then incubated with affinity-purified anti-VHA-11 antibodies. The amounts of the yeast and C. elegans lysates used were 30 and 10 μg, respectively. The solid arrow indicates the position of the VHA-11 protein (40 kDa). The open arrowhead indicates a nonspecific band for the yeast lysates. B-D, immunochemical localization of VHA-11 in C. elegans. An adult worm (B), embryos (200 cells) at the cleavage stage (C), and embryos at the comma stage (D) were fixed and then incubated with the affinity-purified anti-VHA-11 antibodies. VHA-11 was significantly observed in H-shaped excretory cells in the anterior part of an adult worm (B), consistent with a previous report involving the GFP reporter gene (9). The arrow indicates the position of the nucleus of an H-shaped excretory cell. In C and D, VHA-11 was localized in dot-like structures around the nuclei and also detected in the cytoplasm. Arrowheads indicate the dot-like structures with which VHA-11 was densely associated. Scale bars indicate 50 μm (B) or 10 μm (C, D). | |||
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| Name | F1.large.jpg | ||||
| Depict | Expr_pattern | Expr951 | |||
| Anatomy | WBbt:0005792 | ||||
| WBbt:0005812 | |||||
| Cellular_component | GO:0005634 | ||||
| GO:0005737 | |||||
| GO:0043229 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2000 | ||||
| Article_URL | DOI | id | 10.1074/jbc.M002756200 | ||
| Journal_URL | TheJournalofBiologicalChemistry | ||||
| Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
| Reference | WBPaper00004341 |
