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WBPicture0000008270DescriptionFigure 4. Expression pattern of the UNC-63::GFP and the LEV-1::GFP constructs. A, structure of the injected GFP constructs. B, composite image of L3/L4 transgenic larvae stably transformed with UNC-63::GFP. The GFP signal is observed in a large number of neurons in the lumbar ganglia (lg), pre-anal ganglia (pag), head ganglia (hg), and ventral nerve cord (arrows indicate motor neuron cell bodies). Scale bar = 50 μm. C, UNC-63::GFP signal is shown in a subset of neurons in the anal ganglia. No expression is observed either in the sphincter muscle cell or in the anal depressor muscle. Scale bar = 5 μm. D, dorsal view of a young adult animal. All the body wall muscle cells, visible as large trapezoidal cells (example indicated by arrow), express UNC-63::GFP. A dark strip separates the left and right quadrants. Scale bar = 50 μm. E, four neurons (see arrows) of the posterior lateral ganglion express UNC-63::GFP. Scale bar = 5 μm. F, the UNC-63::GFP signal is expressed in some of the vulval muscle cells indicated by arrows. Scale bar = 10 μm. G, the lev-1::GFP construct was injected into the lev-1 mutant (allele e211). GFP expression is observed from embryo to the adult stage, where signal is found in a subset of neurons in the ventral nerve cord, indicated by arrows. H, LEV-1::GFP expression in body wall muscles. Scale bar = 50 μm.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2004
Article_URLDOIid10.1074/jbc.M404370200
Journal_URLTheJournalofBiologicalChemistry
Publisher_URLTheAmericanSocietyForBiochemistryandMolecularBiology
ReferenceWBPaper00024530