WormBase Tree Display for Picture: WBPicture0000008355

WBPicture0000008355 Description: Figure 5. SMF-1 is expressed in DA neurons. Primary C. elegans cultures expressing GFP in the DA neurons were generated with WT (A-D) or smf-1 mutants (E-H), and fixation was performed as described under Experimental Procedures. Primary cultures were incubated with SMF-1 primary antibodies followed by incubation with Texas Red-conjugated goat anti-rabbit secondary antibodies. Differential interference contrast images of A and E of WT and smf-1 mutant cultures, respectively, are shown. DA neurons from WT and smf-1 mutants expressing GFP driven by the dat-1 promoter B and F, respectively. SMF-1 colocalized with DA neurons in WT animals (C), but not in smf-1 mutants (G). D, overlay of B and C; H overlay of F and G. Images were observed under a Zeiss confocal microscope (Zeiss LSM 510). Scale bar represents 45 μm.
  Name: F5.large.jpg
  Depict: Expr_pattern: Expr8811
    Anatomy: WBbt:0005278
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2009
    Article_URL: DOI id 10.1074/jbc.M109.051409
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00035259