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| WBPicture0000008377 | Description | Figure 5. EGL-43 protein is expressed in the HSN nucleus during migration. Fluorescence photomicrographs of wild-type (A-F) and egl-43(n1079) mutant (G-I) embryos that have been stained with DAPI (blue), an UNC-86 antiserum (green), and an EGL-43 antiserum (red). Each panel presents a left lateral view of the embryo, with the developing tail to the left, the head to the right and the end of the tail and the top of the head both oriented upwards. The right sides are out of focus. (A-C) A wild-type embryo at ~400 min after first cleavage. (A) DAPI staining was used to visualize cell nuclei. The HSN/PHB precursor (large arrow), PHA sensory neuron (arrowhead) and ALN/PLM precursor (small arrow) nuclei are located in the tail. (B) Anti-UNC-86 staining. At this time, only the ALN/ PLM precursor nucleus (small arrow) expresses UNC-86 in the tail (Finney and Ruvkun 1990). (C) Anti-EGL-43 staining. At this time, only the HSN/PHB precursor (large arrow), which will divide to generate an HSN and PHB neuron, and the PHA sensory neuron (arrowhead) express EGL-43 in the tail. (D-F) Wild-type embryo at ~430 min after first cleavage. (D) DAPI staining reveals the positions of the HSN nucleus (large arrow) which has migrated out of the tail, as well as a phasmid neuron nucleus (arrowhead) and an ALN/PLM precursor nucleus (small arrow) in the tail. (E) Anti- UNC-86 staining. Shortly after commencing its anteriorly directed migration, the HSN begins expressing UNC-86 (large arrow; Finney and Ruvkun 1990). (F) Anti-EGL-43 staining. During its migration, the HSN also expresses EGL-43-(large arrow). One of the EGL-43- expressing phasmid neuron nuclei (arrowhead) is also visible. (G-I) egl-43(n1079) mutant embryo at ~430 min after first cleavage. (G) DAPI staining of this egl-43(n1079) mutant embryo shows the position of the HSN nucleus in the tail (large arrow). The phasmid neuron (large arrowhead), ALN/PLM precursor (small arrow) and the sister cell of the ALN/PLM precursor (small arrowhead) nuclei are indicated. (H) UNC-86 expression. The HSN neuron expressing UNC-86 (large arrow) has not migrated from its birthplace in the tail. The nuclei of the ALN/PLM precursor (small arrow) and its sister cell (small arrow) are indicated. (I) Anti-EGL-43 staining. One of the phasmid neurons (large arrowhead) but not the HSN neuron (large arrow) is expressing EGL-43. Scale bar, 5 μm. | |||
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| Name | F5.large.jpg | ||||
| Depict | Expr_pattern | Expr669 | |||
| Anatomy | WBbt:0004365 | ||||
| WBbt:0004366 | |||||
| WBbt:0004757 | |||||
| WBbt:0004758 | |||||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1101/gad.13.4.472 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00003422 |
