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WBPicture0000009511DescriptionFigure 4. Expression of sax-3::GFP. (A) Comma stage embryo. The anterior cells that express sax-3::GFP include developing neurons and epidermal cells. The ventral cells in the midbody are the ventral motor neuron precursors. Much weaker expression is observed in the lateral epidermal cells.(B) L1 stage larva. Expression is high in head motor neurons and interneurons (arrow). Muscles of the head and ventral body also express the transgene (arrowheads). Epidermal expression is absent by this time. No expression is observed in the immature HSN motor neurons.(C) L2 stage larva. The HSN motor neuron is extending a growth cone toward the ventral midline and expresses sax-3::GFP at high levels. Arrowhead denotes muscle expression.(D) L3 stage larva. The HSN axon has reached the ventral midline, but is not yet extending along the longitudinal bundles (arrow). The ventral muscles also express sax-3::GFP (arrowhead).(C) is a confocal image obtained by Tim Yu; all other images are taken using conventional fluorescence microscopy. Anterior is at left and dorsal is up in all panels. Scale bars = 10 μm.
NameF4.jpg
DepictExpr_patternExpr716
AnatomyWBbt:0003679
WBbt:0005113
WBbt:0005409
WBbt:0005733
WBbt:0006761
WBbt:0006830
WBbt:0008390
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year1998
Article_URLDOIid10.1016/S0092-8674(00)80916-2
Journal_URLCell
Publisher_URLElsevier
ReferenceWBPaper00002997