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| WBPicture0000009529 | Description | Figure 5. Expression Pattern of mig-13::GFP. A mig-13::GFP translational fusion was integrated into the genome and examined in wild-type and mig-13(mu31) backgrounds. This fusion fully rescues the BDU and QR-descendant migration defects. All expressing cells show localization to the plasma membrane. The punctate fluorescence seen in (B)-(E) is due to autofluorescent gut granules and not to mig-13::GFP. Anterior is to the left and ventral is down in all panels except for (E), which shows a ventral view. Bars, 10 μm.(A) The earliest expression of mig-13::GFP is seen in the anterior region of the comma-stage embryo. The perimeter of the embryo is outlined in white.(B) The pharyngeal-intestinal valve cells (arrow) show expression throughout all larval and adult stages.(C) Expression in the anterior ventral cord cells (arrows) in an L1 larva. This staining persists throughout all larval and adult stages. Both the cell bodies and their axons display fluorescence, but only the focal plane of the cell bodies is shown.(D) In L1 larvae, DA9 (arrow) is the only cell in the posterior that expresses mig-13::GFP. The circumferential axon that emanates from DA9 is also visible (arrowhead).(E) A ventral view of the cord neurons allows better visualization of their circumferential axons (arrowheads).(F) A lateral view of a circumferential axon in the head of an L1 is indicated by an arrowhead.(G) A schematic summary of the mig-13::GFP expression pattern in an L1 larva is shown in green. The ventral cord neurons project circumferential axons that extend dorsally across the sides of the body. Each axon grows out on either the right (dotted lines) or left (solid lines) side of the animal in a stereotypic manner. Some axons extend directly from their cell bodies, while others emanate from longitudinal extensions along the ventral surface. | |||
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| Name | F5.jpg | ||||
| Depict | Expr_pattern | Expr1335 | |||
| Anatomy | WBbt:0004857 | ||||
| WBbt:0005300 | |||||
| WBbt:0005767 | |||||
| Cellular_component | GO:0005886 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1016/S0092-8674(00)80603-0 | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003594 |
