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WBPicture0000009545DescriptionFigure 4. ZYG-12 Isoforms Localize to Both the Centrosome and Nucleus. Timelapse two-photon images of embryos expressing GFP-tagged ZYG-12 isoforms. Embryos are expressing (A and B) ZYG-12A,B,C; (C and D) ZYG-12A; (E and F) ZYG-12B,C tagged with GFP. (A), (C), and (E) are at the pronuclear migration stage and (B), (D), and (F) are at the centration stage. (C and E) Only one of two centrosomes visible in focal plane. (G) Wild-type embryo at the two cell stage stained with ZYG-12 antibodies. Centrosomes in the anterior cell AB are out of the plane of focus. (H) tba-2 RNAi embryo expressing ZYG-12A,B,C::GFP. All embryos shown are wild-type for the genomic copy of zyg-12. For movie corresponding to Figures 4A and 4B, see Supplemental Movie S3 on Cell website. Bar, 10 μm.
NameF4.jpg
DepictExpr_patternExpr2828
Expr2829
Cellular_componentGO:0005634
GO:0005813
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year2003
Article_URLDOIid10.1016/S0092-8674(03)00985-1
Journal_URLCell
Publisher_URLElsevier
ReferenceWBPaper00006310