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WBPicture0000009589DescriptionFigure 4. Expression of DGK-1. (A) A transcriptional GFP reporter construct (nuIs15) is expressed in many or all neurons, including cells in the in the head and tail ganglia, (B) the ventral cord motor neurons (arrows), axons in the ventral and dorsal nerve cords (VNC and DNC), and in the excretory canals (arrowheads). The full-length gfp::dgk-1 translational fusion (nuIs45) has a similar expression pattern (data not shown). (C) Subcellular localization of the DGK-1 protein was examined with the full-length gfp::dgk-1 translational fusion in nuIs45 animals. This fusion construct rescues the dgk-1 mutant phenotype. In general, GFP::DGK-1 is uniformly distributed throughout the cell body and axons of expresssing cells. In cell bodies, GFP::DGK-1 is localized in a large perinuclear structure (arrows).
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AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year1999
Article_URLDOIid10.1016/S0896-6273(00)80835-1
Journal_URLNeuron
Publisher_URLElsevier
ReferenceWBPaper00003697