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| WBPicture0000009590 | Description | Figure 4. The EGL-8 Protein Is Localized to the Nervous System and near Intestinal Cell Junctions. (A) Indirect immunofluorescence staining of EGL-8 protein in a wild-type C. elegans adult. Note that the nervous system staining is stronger in the head ganglia than in the ventral cord. Confocal cross sections revealed that the lines of staining running along the body are near the luminal membrane of the intestine, and the loop-like structures emanate from the lumen of the intestine. At the end of the intestine, EGL-8 staining becomes brighter and the pattern more complex as the intestinal cells connect to the epithelial rectal cells and the anus is formed (White 1988). The vulval muscle staining is present in a mutant control (see [D] in this figure).(B) Enlarged view of EGL-8 staining in head ganglia. Note staining of neuronal cell somas as well as the axon processes of the nerve ring.(C) EGL-8 intestinal staining is localized near cell junctions. Shown is a confocal image of EGL-8 staining (green) and MH27 staining (red) in the mid-body region of a wild-type L2 stage larvae. Regions of overlap show up as yellow. The centrally located horizontal yellow and orange line is near the junctions formed as pairs of intestinal cells wrap around the lumen (junctions on the other side of the lumen also stain but are not included in this projection). Short vertical yellow and red lines mark the anterior-posterior ends of adjacent intestinal cells. Loops of EGL-8 staining (white arrowheads; see also [A] in this figure) may correspond to the entire region of cell apposition, whereas MH27 only stains the septate junctions.(D) The region of EGL-8 used for antibody production is not made in egl-8 (md1971), which serves as a control for specificity of the EGL-8 antibody. Nonspecific staining of the EGL-8 antibody can be seen in the pharynx, germ cells, vulval region, and cuticle. Double staining with a monoclonal antibody to the neuronally localized CHA-1 protein (data not shown) was used as a control for permeabilization. | |||
|---|---|---|---|---|---|
| Name | F4.jpg | ||||
| Depict | Expr_pattern | Expr1199 | |||
| Anatomy | WBbt:0003679 | ||||
| WBbt:0005772 | |||||
| Cellular_component | GO:0043005 | ||||
| GO:0043025 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1016/S0896-6273(00)80847-8 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003738 |
