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| WBPicture0000009886 | Description | Figure 4. Condensin I Localizes to Meiotic Chromosomes in a Pattern Distinct from Condensin IDC or Condensin II. (A) DPY-27 antibody was used to indicate the localization of condesin IDC, anti-CAPG-1 to indicate condensin I and condensin IDC, and anti-KLE-2 to indicate condensin II. DPY-27 was detected in the nucleoplasm of mature oocytes, but not at other stages of meiosis. CAPG-1 staining indicates that condensin I surrounds chromosomes in prophase then localizes to the interface between homologs (meiosis I) or sister chromatids (meiosis II). In contrast, KLE-2 staining indicates that condensin II localizes to the core of each sister chromatid. Antibody in green, DNA in red, merge in yellow.(B) MIX-1 localization is a hybrid of condensin I and condensin II patterns. MIX-1 (green) partially colocalizes with CAPG-1 (red, representing condensin I) on sperm chromosomes (blue). MIX-1 and CAPG-1 colocalize surrounding chromosomes in meiosis prophase I, between homologs in metaphase I, and between sisters at metaphase II (filled arrows). In addition, MIX-1 localizes to the chromosome core at each stage (open arrows), a pattern resembling condensin II and not shared by CAPG-1. | |||
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| Name | F4.jpg | ||||
| Depict | Expr_pattern | Expr8708 | |||
| Cellular_component | GO:0005694 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2009 | ||||
| Article_URL | DOI | id | 10.1016/j.cub.2008.12.006 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00032450 |
