WormBase Tree Display for Picture: WBPicture0000009937

WBPicture0000009937 Description: (C-E) TRR-1 is expressed throughout development and is localized to nuclei. (C), a 4-cell stage embryo. (D), an L2 larva indicating TRR-1 expression in P4.p, P5.p, and P6.p (arrowheads). (E), a germline oocyte nucleus in an adult hermaphrodite. In (C) and (D), anterior is to the left and dorsal is up. To more clearly depict the weak expression of TRR-1 in somatic postembryonic cells in (D), we improved the signal-to-noise ratio of our initially captured image by adjusting pixel threshold values and applying a Gaussian filter using Adobe Photoshop 5.5. Scale bars, 5 um.(F-H) 4,6-Diamidino-2-phenylindole (DAPI) staining of the same samples shown in (C)-(E). In (E), P4.p, P5.p, and P6.p were identified by their positions and large, DAPI-negative nucleoli. In (F), the anterior-most cell, AB.a, and the dorsal-most cell, AB.p, have condensed mitotic chromosomes, which by comparison with (C) are not sites of concentrated TRR-1 localization. The discrete foci of DAPI staining in the oocyte nucleus shown in (H) indicate condensed meiotic chromosomes, and a comparison with (E) indicates localization of TRR-1 to these chromosomes. Scale bars, 5 um.
  Name: F1_A.jpg
  Crop: Cropped_from: WBPicture0000009936
  Depict: Expr_pattern: Expr2921
    Anatomy: WBbt:0005906
      WBbt:0005994
      WBbt:0008117
      WBbt:0008121
      WBbt:0008125
    Cellular_component: GO:0005634
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2004
    Article_URL: DOI id 10.1016/S1534-5807(04)00065-6
    Journal_URL: DevelopmentalCell
    Publisher_URL: Elsevier
  Reference: WBPaper00013412