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| WBPicture0000010746 | Description | Figure 3. (A) A mixed transgenic array containing multiple copies of the lac operator as well as the full rescuing elt-2::GFP construct pJM86 produce colocalizing fluorescent foci in embryonic gut nuclei, as detected by (Upper Left) antibody against GFP (followed by fluorescently labeled secondary antibodies) and by (Upper Right) exogenously added lac repressor (followed by anti-lac repressor antibody and fluorescently labeled secondary antibodies). Arrows point to several fluorescent foci detected by GFP antibodies that overlap with foci detected by lac repressor binding. The DAPI-stained image of the same field is shown (Lower Left). Each of these three panels represents a 400-nm optical section through a transgenic embryo (8E cell stage). (Lower Right) A three-color merged image in which elt-2::GFP is colored green, lac repressor is colored red, and DNA is colored blue. The yellow area reveals the extensive overlap between nuclear foci detected by elt-2::GFP and lac repressor. Thirty-two such embryos were examined in detail, all of which showed colocalized foci. Parallel treatment of wild-type embryos showed no significant staining (n = 20). The two arrows in the merged image indicate lac repressor binding to condensed metaphase chromosomes. The scale bar represents 10 μm. (B). Binding of elt-2::GFP is specific for its own promoter. Embryos homozygous for two independent transgenic arrays, one containing the full rescuing elt-2::GFP construct pJM86 and the second containing a mixture of lac operator sites and empty plasmid vector, were stained and processed exactly as for A. A 400-nm optical section of a typical embryo is shown, revealing the disposition of (Upper Left) elt-2::GFP, (Upper Right) lac repressor, and (Lower Left) DNA (DAPI). (Lower Right) The three-colored merged image is shown. Foci detected by elt-2::GFP (green) do not overlap with foci detected by lac repressor (red); 33 embryos were examined. | |||
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| Name | F3.large.jpg | ||||
| Depict | Expr_pattern | Expr1216 | |||
| Anatomy | WBbt:0005772 | ||||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | Reprinted with permission from <Journal_URL> <Article_URL>. Copyright (<Publication_year>), <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1073/pnas.96.21.11883 | ||
| Journal_URL | ProcNatlAcadSciUSA | ||||
| Publisher_URL | NationalAcademyofSciencesUSA | ||||
| Reference | WBPaper00003733 |
