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WBPicture0000011000DescriptionFigure 2. Reporters driven by the operon or internal promoter give different expression patterns. (A) The 5' genomic sequence (600 bp) of the first gene of this operon (Y51H1A.3) was used as the 'operon promoter,' while the 880 bp between ing-3 and Y51H1A.3 was used as the 'internal promoter.' (B) GFP and (C) LacZ indicate that the operon promoter was widely expressed in L1 larva, especially in the pharynx. (D) The operon promoter showed GFP expression in the adult vulva, spermatheca (asterisks), neurons, and epidermal cells, among others. (E) Differential interference contrast image corresponding to D. The internal promoter expressed GFP (F) and LacZ (G) in the L1 intestine, the limits of which are indicated by asterisks. The signal between the asterisks in F represents gut auto-fluorescence. Note higher expression in the anterior intestinal cells.
NameF2.large.jpg
DepictExpr_patternExpr8385
Expr8386
AnatomyWBbt:0003679
WBbt:0003681
WBbt:0005319
WBbt:0005733
WBbt:0006748
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2009
Article_URLDOIid10.1534/genetics.107.080515
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00032356