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| WBPicture0000011015 | Description | Figure 5. A rescuing UNC-82::GFP fusion protein is localized near the M-line in body-wall muscle. (A-C) Portions of two body-wall muscle cells from an adult unc-82(e1323) homozygote expressing UNC-82::GFP are shown stained with antibodies against (A) UNC-89/obscurin and (B) GFP. The signals coincide in the merged image (C). The asterisks (A) mark the boundary between the two muscle cells, and the arrows indicate one end of each muscle cell. (D-F) One plane from a z-series in which UNC-82::GFP was detected throughout the depth of the myofilament lattice, as marked by a-actinin (Francis and Waterston 1985; see Figure 1). (G-I) A 1.5-fold embryo, positioned as in Figure 2A, exhibits punctate UNC-82::GFP concentrated near myosin in a single muscle quadrant (arrows). Asterisks mark the posterior tip of the embryo. (J and K) The e1323 embryo on the left (arrow marked '+') expresses UNC-82::GFP, whereas the embryo on the right (arrow marked '-') has lost the unstable transgene. (J) The myosin staining (between asterisks) appears broken and patchy in the mutant without the transgene (-). Anti-GFP signal (K) outside the body-wall muscle quadrants (box in J and K) in only the rescued embryo (+) suggests fusion protein expression in nonmuscle cells. An adult pharynx (L-N) exhibits no overlap between the UNC-89 and UNC-82::GFP signals. (L) UNC-89 signal marks the regions of the single-sarcomere pharyngeal muscle cells that contain thick filaments. The UNC-82::GFP signal (M, arrow in N) appears localized to the marginal cells or to a region of the muscle cell that does not contain thick filaments. (N) The pharynx shown is bent back in the isthmus (i) such that the procorpus (pc) lies alongside the terminal bulb (tb). The antibodies used were anti-UNC-89, MH42; rabbit anti-GFP, Abcam; antimyosin A, 5.6; anti-a-actinin, MH35. Bar, 5 um. | |||
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| Name | F5.large.jpg | ||||
| Depict | Expr_pattern | Expr8836 | |||
| Anatomy | WBbt:0005813 | ||||
| Cellular_component | GO:0031430 | ||||
| Acknowledgment | Template | WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL> | |||
| Publication_year | 2010 | ||||
| Article_URL | DOI | id | 10.1534/genetics.109.110189 | ||
| Journal_URL | Genetics | ||||
| Publisher_URL | GSA | ||||
| Reference | WBPaper00035423 |
