WormBase Tree Display for Picture: WBPicture0000011108

WBPicture0000011108 Description: Figure 1. CLE-1 and NID-1 are concentrated near synaptic zones. A, Schematic representation of an electron micrograph cross section of the ventral nerve cord as viewed from a posterior perspective [based on White et al. (1986), their Figure 18]. From left to right are a muscle cell (M), the left axon bundle (blue open circles), hypodermal ridge (H), a neural cell body (N), the right axon bundle (blue open circles), and another muscle cell (M). The left muscle is projecting a muscle arm (M) over the nerve cord to the right fascicle. Other muscle arms in the section are observed as open black circles closely apposed to the right fascicle. The basal lamina covering the hypodermis and nerve cord is exaggerated in size. The general regions where CLE-1 (red) and NID-1 (green) are enriched are indicated. The remainder of the basal lamina (purple) can have lower levels of both CLE-1 and NID-1. This schematic represents projections of light microscopic data onto an EM scale and is for display purposes only. The gray shaded area indicates the approximate region where synapses are observed in B. A single neuromuscular junction (NMJ) present in the section is indicated, although NMJs can form anywhere along this side of the cord where neurons interface with muscle arms. A distinct basal lamina is present over the muscle cells but is not shown. Scale bar, 200 nm. B, Synaptotagmin-1 (green) and perlecan (red) illustrate the position of synapses (arrows) relative to the muscles. Synapses are not evenly distributed in the region between the two muscle cells but rather are concentrated along the muscle edge. C-K, Immunolocalization of CLE-1 (C-G, green) and NID-1 (H-K, green) relative to the presynaptic protein UNC-17 (red) demonstrating the localization of the basement membrane proteins relative to cholinergic synapses. In all panels, anterior is to the left. Dorsal is up in C,G, and H , although the dorsal surface projects out in D and E and ventral projects out in I-K. Scale bars, 10 μm.C, Significant CLE-1 localization is observed along the axon process of the nerve ring (arrow) and the dorsal and ventral nerve cords (arrowheads). D, CLE-1 appears punctate along the nerve cords; the dorsal nerve cord is shown here. E, Anti-UNC-17 staining illustrates the presynaptic zone of cholinergic synapses along the dorsal cord. F, Merged image of boxed region from D and E. CLE-1 appears to be present along the surface of the cord and heavily concentrated over the synaptic region along the right muscle edge. The same pattern is seen on the ventral nerve cord (data not shown). G, A lateral perspective of the dorsal cord showing that CLE-1 (green) is localized ventral to the synaptic region defined by UNC-17 (red), on the pseudocoelomic face of the cord. H, NID-1 staining is observed on the nerve ring (arrow) and more weakly on the nerve cords (arrowheads) as well as on the pharynx (p) and intestine (i). I, NID-1 localizes between the ventral muscle edges (arrowheads) and nerve cords. A similar pattern is observed on the dorsal cord (data not shown). The more medial staining seen in the top of the boxed region is NID-1 associated with the extracellular mantle of the mechanosensory neuron AVM. J, UNC-17 staining along the right fascicle of the ventral nerve cord. K, Merged image of the boxed regions from I and J. NID-1 is concentrated along the lateral edge of the synaptic region. Staining of the AVM mantle is again seen above the UNC-17 (red) staining.
  Name: F1.large.jpg
  Depict: Expr_pattern: Expr2567
    Anatomy: WBbt:0005829
      WBbt:0006749
      WBbt:0006750
    Cellular_component: GO:0043005
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2003
    Journal_URL: TheJournalofNeuroscience
    Publisher_URL: SocietyofNeuroscience
  Reference: WBPaper00005871