WormBase Tree Display for Picture: WBPicture0000011117

WBPicture0000011117 Description: Figure 1. CeIA-2 GFP reporter gene expression. A, GFP fluorescence image of a transgenic animal harboring the Pida-1 CeIA-2::GFP reporter gene. Very strong expression is detected in the ALA, VC, HSN, and PHC neurons as labeled. Lower-level expression is seen in the uv1 neurosecretory cells of the vulva. Dashed lines indicate the outline of the animal, with the head at the bottom and the tail in the top right corner. The punctate pattern of the Pida-1 CeIA-2::GFP reporter in axons is most easily observed in the solitary lateral axons of ALA, one of which is visible in the focal plane shown. The boxed region of the head expression pattern of the ALA neuron is shown at a higher magnification in the inset. Arrowheads indicate the punctate pattern of GFP observed in most axons of GFP-positive neurons. B, Coincidence of CeIA-2::GFP puncta and Synaptotagmin-labeled synapses. Puncta (arrows) in a lateral ALA axon segment just posterior to the pharynx are shown. These puncta are visualized by CeIA-2::GFP expression (far left) and are revealed as synapses by anti-synaptotagmin antibody staining (far right). The center panel merges the two outside images with anti-synaptotagmin in red and CeIA-2::GFP in green with overlap in yellow. Asterisks mark the ventral and dorsal nerve cords that are slightly out of the focal plane and that are heavily labeled by anti-synaptotagmin staining.
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  Depict: Expr_pattern: Expr2900
    Anatomy (12)
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2004
    Article_URL: DOI id 10.1523/JNEUROSCI.0101-04.2004
    Journal_URL: TheJournalofNeuroscience
    Publisher_URL: SocietyofNeuroscience
  Reference: WBPaper00006469