WormBase Tree Display for Picture: WBPicture0000011189

WBPicture0000011189 Description: FIG.1. Detection of collagen mRNAs synthesized during the development of C. elegant. Either 10 ng of total RNA (lanes 1-4) or 3 ng of poly(A) RNA (lanes 5-12) were electrophoresed through 1.2% agarose, 6 M formaldehyde gels and transferred to nitrocellulose filters. The stage-specific RNAs analyzed were: embryo RNA (lanes 1, 5, and 9), L2-dauer RNA (lanes 2, 6, and 10), dauer-L4RNA (lanes 3, 7, and I I ) , and L4-adult RNA (lanes 4,8, and 12). The filters were probed with nick translated plasmids. To detect the total collagen transcripts the probe was pCol-2a (lanes 1-4) used with low stringency conditions. To detect col-1 specific and col-2 specific transcripts the probes were pCol-lb (lanes5 and 6) and pCol-2b (lanes 9-12), respectively, used with high stringency conditions. The hybridized filters were exposed to XAR-5 film with intensifying screens for 22 h (lanes 1-4) or 72 h (lanes 5-12).
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  Depict: Expr_pattern: Expr609
      Expr610
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1985
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00000746