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WBPicture0000011566DescriptionFigure 7. Distribution of DPY-27 in Early- and Late-Staged Embryos (A-C) Confocal images of a wild-type XX embryo at the 12-cell stage, stained with (A) anti-DPY-27 antibody (false green color) and (6) counterstained with propidium iodide (PI) (false red color). The merged image in (C)was generated by superimposing the images of (A) and (8). DPY-27 staining is diffusely distributed throughout the entire nucleus of each cell in this embryo. This diffuse pattern is indicated by the coincidence of the anti-DPY-27 antibody staining (green) and the propidium iodide staining (red), which results in the yellow color of the merged image. [D-F) Confocal images of a wild-type XX embryo at the >200-cell stage, stained with (D) anti-DPY-27 antibody (green) and (E) propidium iodide (red). In this embryo, the DPY-27 staining is punctate in appearance and has become restricted to a portion of each nucleus, as indicated by the reduced coincidence of the anti-DPY-27 and PI staining in the merged image (F), The regions of yellow in the merged image are much smaller than the red regions representing the nuclei. All scale bars, 10 um.
NameFig7.jpg
DepictExpr_patternExpr4351
Cellular_componentGO:0005634
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year1994
Article_URLDOIid10.1016/0092-8674(94)90255-0
Journal_URLCell
Publisher_URLElsevier
ReferenceWBPaper00002061