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| WBPicture0000011567 | Description | Figure 6. DPY-27 Antibody Staining of Wild-Type and dpy-27(y767) Embryos Fluorescent photomicrographs of wild-type XX embryos stained with anti-DPY-27 antibody made from the first 409 amino acids of DPY-27 (A) and costained with DAPI (B); (C)wild-type XX embryos stained with an antibody to another nuclear protein, UNC-86; (D) dpy-27(y767) embryos stained with the anti-DPY-27 antibody and costained with DAPI (E); (F) dpy-27(y767) embryos stained with anti-UNC-86 antibody. DPY-27 is localized to the nucleus of wild-type XX embryos. The level of DPY-27 is greatly diminished in embryos carrying the null allele dpy-27(y767), thus demonstrating the specificity of the antibody for DPY-27. The ~767 mutation creates an in-frame stop codon at amino acid position 455. The intense staining of dpy-27(y767) embryos with anti-UNC-88 antibodies indicates that reduced staining with anti-DPY-27 antibodies is not caused by a general reduction in protein synthesis or a general increase in protein degradation in these dying embryos. The large reduction in the level of DPY-27 epitope is probably due to the instability of either the peptide or the partially translated mRNA. Scale bar, 20 um. | |||
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| Name | Fig8.jpg | ||||
| Depict | Expr_pattern | Expr4351 | |||
| Cellular_component | GO:0000228 | ||||
| GO:0000805 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1994 | ||||
| Article_URL | DOI | id | 10.1016/0092-8674(94)90255-0 | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00002061 |
