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WBPicture0000011577 Description: Fig. 7. Specificity of cellular expression of the tba-l::lacZ fusion gene during development. The fusion gene expression pattern during embryogenesis: scale bar is 20 microns. (a) In the late gastrulation stage embryo, staining of a subset of cells; (b) staining in the morphogenesis stage embryo, showing an increase both in number of cells and intensity of staining; (c) late embryogenesis, during two-fold stage, showing reduction in the number and intensity of cells stained. All stained cells appear to be the descendants of AB founder cell, but because of the clustering they have not been identified individually; (d) post-embryonic staining of a late L1 larva, a large arrowhead showing staining of the QL precursor cell, that generates among others, PVM touch receptor neurons on the left side, in the posterior half of the animal; (e) the same animal as in (d) stained with the nuclear dye DAPI. Stained nuclei in the ventral nerve cord correspond to the motor neuron class DA1-DA9. DA1 is located in the retro-vesicular ganglion, DA2-DA7 along the ventral cord, and DA8 and DA9 are located in the pre-anal ganglion. The cell bodies of the posterior touch receptor neurons PLML and PLMR in the pair of lumbar ganglia on the left and right side in the tail region are also stained. A few additional neurons in the head stain, but these remain unidentified; (f) staining in an adult hermaphrodite, including the set of 38-39 motor neurons along the ventral nerve cord, that have been tentatively identified as DA, DB, VA, and VB classes, based on their cell body position along the ventral cord [38]. Intense staining is seen in and behind the nerve ring in a few neurons in the head region (ventral ganglion', retro-vesicular ganglion, and lateral ganglia), and in the tail region (pre-anal ganglion, dorso-rectal ganglion, and a pair of lumbar ganglia). The ventral nerve cord appears twisted, as is the whole body because of the rol-6 gene as the cotransformation marker; (g) Anterior touch receptor neuron ALMR, the cell body marked by a large arrowhead, located in the anterior half of the adult animal; an anteriorly directed axonal process emanates from the cell body and extends anteriorly to the nerve ring, where it makes a branch that synapses into the nerve ring; on the left side a similar ALML neuron and its process is seen (not shown); (h) anteriorly directed process (shown here by three small arrowheads) of the PLML neuron located in the left lumbar ganglion; a small posteriorly directed process from the cell body extends posteriorly into the tail spike (not in focus), similar staining of the PLMR neuron and its axonal processes is observed on the right side (not shown); (i) the large cell body of the PVM neuron in an adult hermaphrodite, shown by a large arrowhead, a ventrally directed axonal process grows ventrally and on meeting the ventral cord extends anteriorly along the axonal processes of the ventral cord (small arrowheads pointed upward); the anteriorly directed PLML process along the lateral cord is shown by small arrowheads pointing downward.
  Name: Fig7.jpg
  Depict: Expr_pattern: Expr719
    Anatomy (18)
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1995
    Article_URL: DOI id 10.1016/0167-4781(95)00028-F
    Journal_URL: BiochimicaetBiophysicaActa
    Publisher_URL: Elsevier
  Reference: WBPaper00002176