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| WBPicture0000011625 | Description | Figure 9. ADM-1 is expressed in the boundaries among embryonic cells, hypodermis, and sheath cels associated with sensilla. Immunofluorescence micrographs of different developmental stages of C.elegans stained with17B7 monoclonal antibody (A, C, and E) and affinity-purified rabbit anti-ADM-1 antibodies raised against peptide bp2 (F). (A)Most embryonic cells show punctate 17B7 immunoreactivity between the cell boundaries.(B) Same focal planes as the embryo in A stained with DAPI showing some of the 500 nuclei. (C) Punctate staining between blastomeres in a -30 cell embryo. (D) DAPI staining for the embryo in C. (E)Comma stage embryo showing staining between hypodermal cells in the ventral side (arrow)and between cells of the head. (F)Two sheath cells of the aphids at each side of the buccalcavity of a second stage larva L2; at the tip of the head (top left corner) is punctate staining corresponding to the sheath cells of the four cephalic, six outer labial,and six inner labial sensilla (Wardetal.,1975).The staining is not nuclear and corresponds to the position of the sensilla.Some punctate staining is seen in the processes and hypodermis. The serum used was affinity purified as described (see MATERIALS AND METHODS). Similar staining was obtained with other affinity-purified fractions that had a high titer using an ELISA and specifically recognized peptides bp2 or bp3 (Figure2). (G) Same field as in F showing the adherens junctions of epithelial cels in the hypodermis and pharynx stained with MH27. A-E were obtained with an Axioskop Zeiss microscope and F-C with a MRC-600 (Bio-Rad) confocal laser microscope. Bars, 10 ,um. | |||
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| Name | FigS.jpg | ||||
| Depict | Expr_pattern | Expr594 | |||
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| Anatomy | WBbt:0005116 | ||||
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| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1996 | ||||
| Article_URL | DOI | id | 10.1091/mbc.7.12.1877 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00002626 |
