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WBPicture0000011744DescriptionFig. 4. Northern blot analysis and whole-mount in situ hybridization. (A) mRNA levels of csq-1 at different developmental stages were assayed. Equal amounts (1 ug) of poly(A)+ RNA were loaded in each lane and hybridized with a 32P-labeled csq-1 cDNA probe. A transcript of approx. 1.5 kb is observed in all three lanes. (B-E) In situ localization of csq-1 RNA at different developmental stages, viewed under a light microscope (Nomarski optics). Antisense probes show csq-1 expression (arrows) along the mid-body line in the twofold stage embryo (B), in body-wall muscle cells in the larva (C) and in the adult (D,E). (F) DAPI stained nuclei of the muscle cells showing csq-1 expression. Arrows indicate cytoplasmic signals and arrowheads indicate nuclei of body-wall muscle cells. Bars, 50 um.
NameFigC.jpg
DepictExpr_patternExpr1103
Expr1104
AnatomyWBbt:0005813
Cellular_componentGO:0005634
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Link to Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2000
Journal_URLJournalofCellScience
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00004468