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| WBPicture0000011880 | Description | Figure 2. ATX-3 is expressed throughout development and in several cell types. A) Strain BC14364 that has an integrated transgene containing the GFP gene controlled by the 2.0 kb upstream region of atx-3 gene. Expression was observed in late embryos, eggs, larvae, and adults (a, c, d). The BC14364 strain revealed a strong signal in spermatheca (f) and ventral cord (e). It also shows prominent staining of the pharynx (b). Ph, pharynx; cb, cell body; sp, spermatheca. White bar = 0.2 mm, dashed bar = 0.05 mm. B) Semiquantitative polymerase chain reaction (PCR) showing that atx-3 is expressed in all developmental life stages and more abundantly in adult animals. The gel picture shows a representative PCR and the graph shows normalized ratios of atx-3 expression using act-1 as housekeeping gene. C) ATX-3::GFP colocalizes with 4',6'-diamidino-2-phenylidole (DAPI) in the nucleus. Arrows point to one of the cells where there is nuclear localization of ATX-3. a) GFP filter images, b) DAPI filter, c) GFP and DAPI merged pictures, d) schematic GFP and DAPI merged picture, where the DAPI filter was marked as red instead of blue to enhance colocalization. D) Cellular fractioning and Western blot using our anti-303JSP antibody revealed that ATX-3 is present in the nucleus but is more abundant in cytosol (S100) in wild-type animals. Middle panel shows an anti-alpha-tubulin Western blot to prove there is no cytosolic contamination in the nuclear extract; the lower panel shows anti-histone H3 Western blot. The loaded amount of nuclear extract was 2.5-fold more than the cytosolic in order to detect the nuclear fraction of ATX-3. | |||
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| Name | FigB.jpg | ||||
| Depict | Expr_pattern | Expr4767 | |||
| Anatomy | WBbt:0003681 | ||||
| WBbt:0005319 | |||||
| WBbt:0005829 | |||||
| Cellular_component | GO:0005634 | ||||
| GO:0005829 | |||||
| Acknowledgment | Template | Image used with permission from The FASEB Journal (http: | |||
| Publication_year | 2007 | ||||
| Article_URL | DOI | id | 10.1096/fj.06-7002com | ||
| Journal_URL | FASEBJournal | ||||
| Publisher_URL | FederationAmerSocExpBiol | ||||
| Reference | WBPaper00029047 |
