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| WBPicture0000012257 | Description | (A) Alignment of the C-terminal region of TM III of ionotropic glutamate receptors. Shown are representative receptors of the non-NMDA and NMDA classes from C. elegans GLR-1 (1), rat GluR1 (2), rat GluR5 (3), Drosophila GluR1 (4), rat NR1 (5), rat Ka1 (6), rat delta2 (7), and mouse delta2 (8). The conserved alanine found in TM III is shown in red. This residue is mutated to a threonine (shown in green) in the mouse Lurcher strains Lc and LcJ. We have engineered an identical substitution into C. elegans GLR-1(A/T).(B and C) GFP expression in transgenic strains. Under control of the glr-1 promoter, larval transgenic strains expressed chimeric proteins that contained GFP fused to the N terminus of wild-type GLR-1 (B) or GLR-1(A/T) (C). Images acquired with confocal microscopy show that transgenic strains expressed the GLR-1(A/T)::GFP fusion protein in the appropriate neurons and that these neurons show no evidence of gross morphological abnormalities. | |||
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| Name | F2_A.jpg | ||||
| Crop | Cropped_from | WBPicture0000012256 | |||
| Depict | Anatomy | WBbt:0003679 | |||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1016/S0896-6273(00)80849-1 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003743 |
