WormBase Tree Display for Picture: WBPicture0000012458

WBPicture0000012458 Description: Muscle abbreviations: DSP: dorsal spicule protractor; VSP: ventral spicule protractor; ADP: anal depressor. To obtain the expression pattern of unc-29 in the C. elegans male, we initially fused to the YFP gene a 2.2 kb sequence, which includes a 1.3 kb of sequence upstream of the unc-29 start codon and the genomic sequence of the gene up to the second intron (Punc- 29:YFP). This construct is expressed in the anal depressor muscle and the spicule protractor muscles in the male spicule circuit, and it is also expressed in the body wall muscles, some ventral cord neurons and some head neurons. This is consistent with the previous published expression pattern of unc-38, which encodes an a-subunit that forms the L-AChR with unc-29 (Liu et al. 2007). Nonetheless, the unc-29 genomic sequence also contains important information to facilitate transcription of the gene. Therefore, the expression construct that contained the unc-29 genomic sequence(Punc-29:unc-29::YFP) provided a broader expression pattern than Punc-29:YFP. (A-D) Expression of Punc-29:YFP (yellow) and Pgar- 3:CFP (cyan) imaged from the tail of a same L4 larval male. The two images are merged to show that unc-29 is not expressed in the SPC neurons where gar-3 is expressing (C). The Nomarski image of the tail is also shown (D). Scale bar, 10 um. (E, F) Expression of Punc-29:YFP in the male tail. Scale bar, 20 um.
  Name: FigO_A.jpg
  Crop: Cropped_from: WBPicture0000012457
  Depict: Expr_pattern: Expr10820
    Anatomy: WBbt:0004292
      WBbt:0005300
      WBbt:0005813
      WBbt:0005826
      WBbt:0006751
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
    Publication_year: 2011
    Article_URL: DOI id 10.1371/journal.pgen.1001326
    Journal_URL: PLoSGenetics
    Publisher_URL: PLoS
  Reference: WBPaper00038246