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WBPicture0000012526 | Description | Fig. 1. Expression of the HMIT family in C. elegans. (A) Expression patterns of transcriptional translational fusion genes. Photographed animals were young adult hermaphrodites. Phmit-1.1::egfp is expressed in the intestine (left panel). Phmit-1.2::egfp is expressed in the amphid sheath glia as indicated by the arrow and the excretory canal cell as indicated by the arrowhead (middle panel). Phmit-1.3::egfp is expressed in the amphid sheath glia as indicated by the arrow and in the intestine as indicated by the arrowhead (right panel). Scale bar = 100 lm. (B) HMIT-1.1::EGFP is localized at the lumenal membrane of the intestine. Arrowheads indicate the lumenal membrane and arrows indicate the basal membrane. A young adult hermaphrodite of a transgenic rescue line tm3301(hmit-1.1);tmEx2407[Phmit-1.1::hmit-1.1::egfp] was photographed. Scale bar = 20 lm. (C) HMIT-1.2::EGFP is localized in the glial cell body (arrow) and the endfoot region (arrowhead), in which cilia of sensory neurons are embedded. The imaged animal was a young adult hermaphrodite of a transgenic rescue line, tm3196(hmit-1.2);tmEx2408[Phmit-1.2::hmit-1.2::egfp]. Scale bar = 50 lm. (D and E) HMIT-1.2::EGFP is localized at the lumenal membrane of the proximal canal (D) and the basal membrane of the distal canal (E). This localization pattern was exhibited in all the analyzed young adult hermaphrodites of a transgenic rescue line tm3196;tmEx2732[Phmit-1.2::hmit-1.2::egfp, Phmit-1.2::DsRed] (n = 5). DsRed fluorescence completely fills the space between the canal lumen (arrowheads) and the basal membrane (arrows). The asterisk indicates the excretory canal cell body. Scale bar = 10 um. (F) Schematic drawing of the tissue distribution and subcellular localization of HMIT family in C. elegans. | |||
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Name | FigC.jpg | ||||
Depict | Expr_pattern | Expr9308 | |||
Expr9309 | |||||
Expr9310 | |||||
Expr9311 | |||||
Expr9312 | |||||
Expr9313 | |||||
Anatomy | WBbt:0005772 | ||||
WBbt:0005775 | |||||
WBbt:0006754 | |||||
WBbt:0006755 | |||||
Cellular_component | GO:0016020 | ||||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
Publication_year | 2011 | ||||
Article_URL | DOI | id | 10.1016/j.bbrc.2011.06.001 | ||
Journal_URL | BiochemicalandBiophysicalResearchCommunications | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00038529 |