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WBPicture0000012619DescriptionFig. 3. Cah-4 codes for both cytoplasmic and nuclear carbonic anhydrase isoforms. Representative DIC (A-C) and fluorescent maximum projection confocal micrographs (D-F) of transgenic strains expressing promoter::ORF::GFP fusions, as indicated. Where possible, arrows have been used to indicate the relevant cells and cell groups. (A, D) The intra-exonic cah-4a promoter drives GFP expression throughout the nervous system, as well as in head muscle cells. (B, E) The cah-4b promoter is active in a variety of tissues, including the excretory cell, body wall muscle, hypodermis, seam cells, and the intestine, but is not expressed in neurons. These cells comprise most of the body architecture and are thus not indicated by individual arrows. The CAH-4B::GFP fusion protein resides in the cytoplasm. (C, F) The CAH-4A::GFP fusion protein is targeted primarily to the nucleus, as is most readily apparent in the head muscle cells. HMC, head muscle cell; DNC, dorsal nerve cord; EC, excretory cell; Hyp, hypodermal cells; Int, intestine; VNC, ventral nerve cord.
NameFigE.jpg
DepictExpr_patternExpr9943
Expr9944
AnatomyWBbt:0003675
WBbt:0004697
WBbt:0005772
WBbt:0005812
WBbt:0006749
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2012
Article_URLDOIid10.1016/j.bbamcr.2011.12.014
Journal_URLBiochimicaetBiophysicaActa
Publisher_URLElsevier
ReferenceWBPaper00040634