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| WBPicture0000012791 | Description | Figure 2. MFAP-1 is a nuclear protein expressed in multiple tissues. (A) A 2.5 kb 5' promoter region upstream of the start codon of mfap-1 was used to drive the expression of a transcriptional fusion GFP reporter in transgenic animals. The tissues in which the GFP reporter was expressed are labeled. This figure shows a montage of two photographs, which were pseudo-colored from a gray-scale image to a green-scale image using Adobe Photoshop to reflect the level of GFP expression. (B) Transgene experiments were performed as described in Materials and Methods. The C. elegans MFAP-1::GFP fusion protein, when expressed in body-wall muscles using a myo-3 promoter in either wild-type or mfap-1(n4564 n5214) animals of mixed developmental stages, was exclusively localized in nuclei (left and middle panels). Similarly, human MFAP-1::GFP fusion protein, when expressed in the body-wall muscles of mfap-1(n4564 n5214) animals, was also exclusively localized in nuclei (right panels). Lower panels show typical nuclear GFP signals in adult transgenic animals. Genetic backgrounds and transgenes are indicated. | |||
|---|---|---|---|---|---|
| Name | FigF.jpg | ||||
| Depict | Expr_pattern | Expr10144 | |||
| Anatomy | WBbt:0003681 | ||||
| WBbt:0005772 | |||||
| WBbt:0005813 | |||||
| WBbt:0005821 | |||||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2012 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1002827 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00041328 |
