WormBase Tree Display for Picture: WBPicture0000013196
expand all nodes | collapse all nodes | view schema
| WBPicture0000013196 | Description | Figure 5. FLP-18 expression is selectively increased in the cholinergic motor neurons in the acr-2(gf) background. (A-B) Ventral nerve cord expression of Pflp-18::flp-18::SL2::gfp in wild type (N2) and acr-2(gf) background, respectively. Increased fluorescence intensity and cell body expression is seen in the ventral cord in the acr-2(gf) background. Arrows point to the ventral nerve cord posterior to the vulva, and arrowheads point to cell bodies. Scale bar = 25 mm. Two Pflp-18::flp-18::SL2::gfp transgenic lines, juEx4062 and juEx4073, were tested. Images from juEx4073 are shown. (C) Quantification of average fluorescence intensity in the ventral nerve cord posterior to the vulva. Mean fluorescence intensities are shown. N= 37 (wild type), = 38 (acr-2(gf)). ***: p,0.001 by student's t-test. Error bars indicate SEM. (D) Quantification of the number of cell bodies in the ventral cord with visible GFP expression. ***: p,0.001, **: p,0.01 by Student's t-test. Each dot indicates quantification from one animal. Means are indicated by lines. Error bars indicate SEM. Two transgenic lines were tested. (E-H) Identification of the cells showing up-regulation of Pflp-18::flp-18::SL2::gfp in the acr-2(gf) background. (E) Co-expressing mCherry in GABAergic (Pttr-39) motor neurons did not show overlap with Pflp-18::flp-18::SL2::gfp. (F) Expression of mCherry in B-type (Pacr-5) cholinergic motor neurons overlapped extensively with Pflp-18::flp-18:SL2::gfp expression. (G) mCherry expression in A-type (Punc-4) cholinergic motor neurons mostly did not overlap with Pflp-18::flp-18::SL2::gfp expression. (H) Quantification of the number of cell bodies that showed overlapping expression of Pflp-18::flp-18::SL2::gfp and Pacr-5::mCherry or Punc-4::mCherry in F-G. Each dot indicates quantification from one animal. Means are indicated by lines. Error bars indicate SEM. | |||
|---|---|---|---|---|---|
| Name | FigE.jpg | ||||
| Crop | Crop_picture | WBPicture0000013197 | |||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2013 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1003472 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00042376 |
