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WBPicture0000013337DescriptionFigure 10.-LIN-23 is a broadly expressed cytoplasmic protein. (A) Schematic of expression constructs. Q12Stop corresponds to the mutation in the rh294 allele and P610S to the ot1 allele. A question mark indicates potential alternative start codons. (B) Expression of the lin-23 transcriptional reporter (lin-23prom::gfp) and a lin-23 translational reporter (lin-23::gfp). LIN-23::GFP first reveals expression during gastrulation and is strictly restricted to the cytoplasm in embryonic cells and throughout the cell body and axon in the ventral cord (arrows point to left and right cord) in adult animals. (C) Comparison of expression of wildtype and mutated forms of LIN-23::GFP in an adult wild-type background [slightly twisted due to the rol-6(d) injection marker]. All constructs were injected at the same concentration (20 ng/l). At least three lines were scored per construct. Speckles are gut autofluorescence. Arrows point to expression in head ganglia.
NameFigJ.jpg
DepictExpr_patternExpr12239
Expr12240
AnatomyWBbt:0005829
Cellular_componentGO:0005737
GO:0030424
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2004
Article_URLDOIid10.1534/genetics.166.3.1253
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00006497