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WBPicture0000013452DescriptionFigure 3 Expression patterns of mod-1 and ser-4 reporter transgenes are mostly distinct. Shown are confocal micrographs of transgenic animals in which the mod-1 promoter drives expression of the red fluorescent protein mCherry and the ser-4 promoter drives expression of green fluorescent protein (GFP). Red or green fluorescence and a merge of both superimposed on a brightfield image of the animal are shown, as indicated. (A-C) Adult head region. Arrow indicates AIB, the only cell type that reliably coexpressed both reporters. Arrowhead indicates the serotonergic NSM neurons. The double-labeling pattern seen in C is shown in more detail in File S3, a movie of a rotating threedimensional confocal image of the head region. (D-F) Adult tail region. (G) mod- 1:: mCherry fluorescence in an L3 larva superimposed on a brightfield image of the animal. Arrows point to mCherrylabeled ventral cord neurons (VNC), while brackets indicate labeled bodywall muscles (bwm). (H) mod-1::mCherry expression in adult head. The white background used allows weak red fluorescence in body-wall muscle to be visualized. Bracket indicates an individual muscle cell. (I-K) Lower magnification images of a young adult expressing mod-1:: mCherry and an unc-47::GFP transgene that labels GABAergic neurons. Arrowheads in K point to the double-labeled GABA neurons of the ventral nerve cord that are most visible in this individual. Bars, 20 um.
NameFig3.jpg
DepictExpr_patternExpr10553
Anatomy (12)
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2012
Article_URLDOIid10.1534/genetics.112.142125
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00041535