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| WBPicture0000013586 | Description | A) Confocal images showing the Paakg-5::GFP expression pattern in 1-day old hermaphrodites. The Paakg-5::GFP transgene was created using the fusion method of PCR to 'stitch' together 1.85 Kb of promoter taken directly upstream of the transcriptional start site, GFP and the unc-54 39UTR [79]. This PCR product was then introduced as an extra-chromosomal array into N2 worms and two independent transgenic strains were isolated for each gene. (i) Whole worm expression pattern. (ii) Paakg-5::GFP is expressed in the female gonad sheath cells (GonSh), vulva epithelium (VlvE) and neurons (VlvN), ventral cord neurons (VC) and excretory cell (Exc). (iii) It is also seen in the spermatheca (Sperm) and epithelial seam cells (Seam). (iv) In addition to the excretory cell (Exc), Paakg-5::GFP displays strong expression levels in the pharyngeal epithelia (PhxE), neurons (PhxN), some ring neurons (RingN) and sensory neuron (SensN) termini. (v) In the tail, Paakg-5::GFP signal mostly localizes to the pre-anal ganglion (RectN), rectum epithelium (RectE), intestinalrectal valve (RectV) and phasmid support cells (PhaSh, PhaSc). As seen along the whole worm, it is also clearly expressed in the seam cells (Seam), intestine (Int) and excretory cell arms (Exc). Table S10 compares expression of Paakg-5::gfp with other AMPK subunits. | |||
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| Name | S6_A.jpg | ||||
| Crop | Cropped_from | WBPicture0000013585 | |||
| Depict | Expr_pattern | Expr11583 | |||
| Anatomy (16) | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2014 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1004109 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00044832 |
