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WBPicture0000013779DescriptionFigure 3 Co-expression of innexin proteins in the adult gonad. (A) Antibodies specific to the carboxyl termini of INX-8 and closely related INX-9 detect expression in the DTC and somatic sheath cells, as seen in a composite of a dissected gonad arm. Expression is absent or much reduced in the mitotic zone (M) and in areas where no somatic cell overlies the germ line. (B-H) Colocalization of innexins throughout the gonad. In B-H, the approximate corresponding position in the gonad arm is compared to A. (B-D) Colocalization in the region of the DTC of INX-9::GFP and INX-22 (B), INX-21 and INX-22 (C), and INX-14 and INX-22 (D). A medial focal plane of a dissected gonad (D) shows that INX-14 and INX-22 appear to associate with DTC processes that intercalate between germ cells. (E) INX-8::GFP and INX-22 colocalization in the region of the distal end of sheath coverage over germ cells. (F) INX-14 and INX-22 colocalization among pachytene-stage germ cells. (G) INX-8/9 and INX-21 colocalization in the loop region where germ cells leave the pachytene stage. (H) INX-14 and INX-22 colocalize in the proximal arm. M, mitotic region; T, transition zone; P, pachytene. Images in D and F were taken using an apotome adaptor. Bars, 20 um.
NameFigC.jpg
DepictExpr_patternExpr11974
Anatomy (11)
Cellular_componentGO:0005921
Life_stageWBls:0000002
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2014
Article_URLDOIid10.1534/genetics.114.168815
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00045691