WormBase Tree Display for Picture: WBPicture0000013881

WBPicture0000013881 Description: Figure 1. Effects of expression of vps-4(E219Q) (VPS-4 DN) under the odr-10 promoter. A. Image of the head of an animal to illustrate the distribution of ODR-10::GFP fluorescence in an AWA neuron of an animal expressing VPS-4 DN under the odr-10 promoter. Animals were scored for having no visible fluorescence, fluorescence in the cilia, fluorescence in the cell body (arrowhead), with or without ciliary fluorescence (arrow). Red fluorescence is from the injection marker, Pttx-3::dsRed (asterisks). Image acquired at 630X magnification. B. Localization of ODR-10::GFP in the AWA neurons of adult hermaphrodites. Bar graph shows the relative distribution of phenotypes under different conditions. Numbers in the columns indicate the number of animals observed. Data are significant with p << 0.001 using a Chi-square contingency test, Chi-Square value = 123.82, 6 degrees of freedom. No assumptions were violated. C. Effects of different conditions on chemotaxis toward diacetyl, compared to ethanol control for young adult hermaphrodites; odr-10 (ky32) is shown as a negative control. Animals in chemotaxis experiments did not express ODR-10::GFP. Box and whisker plots show the results of 12-13 independent trials. Median chemotaxis indices were wild type (N2) = 0.85, wild type (N2), starved = 0.8, VPS-4 DN = 0.81, VPS-4 DN, starved = 0.7, odr-10 (ky32) = 0.08. Boxes bound the 1st and 3rd quartiles and circles represent outliers of greater than 1.5 times either quartile. Chemotaxis indices were calculated using the following expression: ((animals at chemoattractant)-(animals at control))/total animals on the plate. * p<0.05 compared to wild type, Tukey-Kramer post-test.
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  Acknowledgment: Template: Reprinted from <Journal_URL>
    Publication_year: 2018
    Article_URL: DOI id 10.17912/gnyw-v322
    Journal_URL: Micropublication:biology
    Publisher_URL: Micropublication
  Reference: WBPaper00054955