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| WBPicture0000014088 | Description | Figure S2. RIC-7 and mitochondria localization, related to Figure 2. (A) Tagged RIC-7 (RFP::RIC-7) was made by combining the cDNA for exons 1-10 with the genomic sequence for exons 11 through the 3'UTR. The 10th intron was shortened in order to remove the coding region for F58E10.7. RFP flanked by linker sequences was inserted at the N-terminus. The construct was expressed in GABA neurons using the unc-47 promoter. (B) When inserted as a single-copy transgene, RFP::RIC-7 is distributed diffusely in the GABA motor neuron cell body (arrowhead) and axons. Scale bar = 10 um. (C) Mitochondria are absent in axons of the dorsal nerve cord in ric-7(n2657) mutants. GABA motor neuron axons are labeled with soluble RFP and mitochondria in the GABA neurons are labelled with mito::GFP. Scale bar = 10 um. (D) Mito::citrine was expressed in the hypodermis under the dpy-7 promoter. Similar structures were observed in the wild type and ric-7 mutants. (E) Mito::citrine was expressed in the body wall muscles under the myo-3 promoter. Muscle mitochondria form a similar network in ric-7 mutants and the wild type. Scale bar = 5 um. | |||
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| Name | FigS2.jpg | ||||
| Crop | Crop_picture | WBPicture0000014089 | |||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2014 | ||||
| Article_URL | DOI | id | 10.1016/j.cub.2014.02.025 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00045008 |
