WormBase Tree Display for Picture: WBPicture0000014175

WBPicture0000014175 Description: Fig 2. TBC-2 colocalizes with RAB-10 and CNT-1 on endosomes. All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins specifically in intestinal epithelial cells. (A-A'') GFP-TBC-2 colocalizes well with tagRFP-RAB-10. Arrowheads indicate endosomes labeled by both GFP-TBC-2 and tagRFP-RAB-10. (A''') Magnified image of A'' isdesignated by rectangular outline. (B-B'') GFP-TBC-2 colocalizes extensively with tagRFP-RAB-10(Q68L), a predicted constitutively active form of RAB-10. Arrowheads indicate endosomes labeled by both GFP-TBC-2 and tagRFP-RAB-10(Q68L). (B''') Magnified image of B'' is designated by rectangular outline. (C-C'')GFP-TBC-2 colocalizes well with CNT-1-MC. Arrowheads indicate endosomes labeled by both GFP-TBC-2 and CNT-1-MC. (C''') Magnified image of C'' is designated by rectangular outline. In each image autofluorescent lysosome-like organelles appear in blue in all three channels, whereas GFP appears only in the green channel and RFP shows up only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (Scale bar: 10 um) (D) Pearson's correlation coefficient for colocalization of GFP-TBC-2 with tagRFP-RAB-10, tagRFP-RAB-10(Q68L), and CNT-1-MC. n = 6. Error bars represent SEM. *P<0.05 (student's t test).
  Name: FigH.jpg
  Depict: Expr_pattern: Expr12475
    Cellular_component: GO:0005769
      GO:0005770
      GO:0090654
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
    Publication_year: 2015
    Article_URL: DOI id 10.1371/journal.pgen.1005514
    Journal_URL: PLoSGenetics
    Publisher_URL: PLoS
  Reference: WBPaper00048576