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WBPicture0000014176DescriptionS2 Fig. TBC-2 colocalizes partially with RAB-5 and RAB-7, and does notcolocalize with EHBP-1. All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins specifically in intestinal epithelial cells. (A-A'') GFP-TBC-2 colocalizes partially with tagRFP-RAB-5 on punctate endosomal structures. (B-B'') GFP-TBC-2 colocalizes partially with tagRFP-RAB-7. (C-C'') GFP-TBC-2 displays little colocalization with EHBP-1-MC. In each image autofluorescent lysosome-like organelles appears in blue in all three channels, whereas GFP appears only in the green channel and RFP shows up only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (Scale bar: 10 um) (D) Pearson's correlation coefficient for colocalization of GFP-TBC-2 with tagRFP-RAB-5, tagRFP-RAB-7, and EHBP-1-MC. n = 6. Error bars represent SEM.
NameFigS2.jpg
DepictExpr_patternExpr12475
Cellular_componentGO:0005769
GO:0005770
GO:0090654
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2015
Article_URLDOIid10.1371/journal.pgen.1005514
Journal_URLPLoSGenetics
Publisher_URLPLoS
ReferenceWBPaper00048576