WormBase Tree Display for Picture: WBPicture0000014295

WBPicture0000014295 Description: (A and A0) PFK-1.1::eGFP (pseudocolored magenta) localization in NSM after approximately 10 min (A) and 30 min (A0) of exposure to hypoxic conditions (see Supplemental Experimental Procedures). Clustering dynamics of PFK-1.1 vary between individual animals, but clustering of PFK-1.1 is observed for most animals within 10 min of being exposed to hypoxic conditions. Here we filmed a neuron with slow clustering dynamics to capture the time-lapse image. Cell body of NSM neuron is indicated by asterisk. The time-lapse images of the inset in (A) and (A0) (taken at 1 min intervals) are displayed below. See Movie S2 for time-lapse movie.(B and B0) Pixel fluorescence values for the first inset in the montage (B) (yellow trim in bottom row) and the last inset (B0) (orange trim in bottom row). (C-E) PFK-1.1::eGFP (pseudocolored magenta) and mCherry::RAB-3 (pseudocolored green) simultaneously visualized in AIY interneurons. PFK-1.1 in AIY after 20 min (C) and 40 min (C0) of hypoxic conditions, and (C0) visualized with synaptic vesicle marker RAB-3 (D); the brightness of (C) was increased more than in (C0) in order to allow for visibility of the diffuse localization of PFK-1.1. Note that PFK-1.1 becomes increasingly clustered to presynaptic sites (denoted with brackets as ''synaptic region'') and is excluded from asynaptic regions (Colon-Ramos et al., 2007; White et al., 1986). Inset in (D) corresponds to zoomed-in (53) image of PFK-1.1 cluster localizing near a pool of synaptic vesicles. Enrichment of PFK-1.1 clustering in the AIY neurite over time in hypoxic treatment was quantified(N = 12) (see Supplemental Experimental Procedures) (E). tinitial is >20 min and tfinal is 20-40 min of hypoxic treatment. Each circle represents an individual animal in the longitudinal study.(F) Quantification of the number of PFK-1.1 clusters in the synaptic region and the asynaptic regions of AIY (N = 15).(G) Pixel fluorescence values of PFK-1.1 and RAB-3 along the neurite for boxed region in (D) to compare the colocalized distribution of PFK-1.1 relative to synaptic vesicle proteins. Left y axis corresponds to PFK-1.1, and right y axis corresponds to RAB-3 pixel fluorescence values.(H) Quantification of the distance between the maximum pixel fluorescence values of PFK-1.1 and the maximum pixel fluorescence values of RAB-3 in AIYneurons (n = 26).
  Name: Fig4_A.jpg
  Crop: Cropped_from: WBPicture0000014294
  Depict: Expr_pattern: Expr12930
    Anatomy: WBbt:0003679
      WBbt:0004446
      WBbt:0004457
    Cellular_component: GO:0043005
      GO:0043025
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2016
    Article_URL: DOI id 10.1016/j.neuron.2016.03.011
    Journal_URL: Neuron
    Publisher_URL: Elsevier
  Reference: WBPaper00049486